HPIMS

SIMULTANEOUS SEPARATION & DETECTION

HIGH PERFORMANCE ION MOBILITY SPECTROMETRY IS A RAPID SEPARATION TECHNIQUE BASED ON THE SIZE AND SHAPE OF MOLECULAR IONS.

HOW DOES IT WORK?

  • A liquid sample ionizes on a needle tip by electrospray ionization
  • Excess solvent evaporates in the desolvation region
  • Solvent-free ions are pulsed through the ion gate into the drift tube
  • Ions travel through the drift tube while colliding with drift gas molecules. Compact ions travel more quickly than bulky ions.
  • A Faraday plate detector at the end of the tube creates an electrical signal, which is converted to a mobility spectrum like the one below:

WHY IMS?

HPIMS is an orthogonal technique to liquid chromatography and mass spectrometry. It provides another dimension to an LC or MS separation, or can be used alone for rapid separation of differently shaped compounds.

THE HPIMS DIFFERENCE

High performance IMS achieves 60+ resolution which is comparable to HPLC/UPLC. This was achieved through Excellims’ innovative drift tube design and years of experience with the technology.

Electrospray ionization (ESI) is paired with HPIMS to analyze nonvolatile compounds. This method ionizes a liquid sample off the tip of a needle by applying a strong voltage to it. ESI opens up HPIMS to a wide range a wide range of applications that were not possible with the older generation of commercial IMS instruments.

TECHNOLOGY – OVERALL ADVANTAGE OF HPIMS

High Performance Ion Mobility Spectrometers (HPIMS) are robust analytical tools with significant advantages when compared to other analytical technologies. HPIMS technology demonstrates a giant leap in performance over conventional IMS and delivers analytical figures of merit; resolution, sensitivity, linear response range and precision comparable to other modern separation and detection methods such as HPLC/UPLC systems. HPIMS is a gas phase analytical technique that separates ions based on their mobilities in milliseconds. The media for separation is neutral gas molecules, typically pure air is used. With novel electrospray ionization sources, HPIMS can be used in place of conventional HPLC for the analysis of non-volatile compounds.

HPIMS delivers resolving power (R = td/t1/2) of 60-120 that is 2-10 times better than other IMS based systems. The separation efficiency is equal to or better than HPLC, but with much greater sample throughput; in addition, the ion mobility based separation is truly orthogonal to HPLC.

ULTRA HIGH ION MOBILITY RESOLUTION

HPIMS delivers resolving power (R = td/t1/2) of 60-120 that is 2-10 times better than other IMS based systems. The separation efficiency is equal to or better than HPLC, but with much greater sample throughput; in addition, the ion mobility based separation is truly orthogonal to HPLC.

HIGH PRECISION

Compared to conventional semi-quantitative IMS, HPIMS demonstrates 2-3% RSD. This improvement enables the HPIMS to be used in many applications where high precision is required; for purity checks, content uniformity and cleaning verification, HPIMS provides on-site analysis capability equal to or better than traditional lab methods. In many applications, HPIMS offers a clear alternative for analysis of non-chromophore chemicals.

EXTENDED LINEAR RESPONSE RANGE

HPIMS delivers nearly three (3) orders of magnitude linear response range for most applications.

COMPARISON CHARTS: HPLC / UPLC / IMS / HPIMS

HPIMS complements HPLC / UPLC by providing rapid analysis with similar or better separation efficiency, low operational cost and high reliability.

HPIMS TECHNIQUE COMPARISON

HPIMS SEPARATION COMPARISON