Separation of biologically relevant isomers on an Orbitrap using High Performance IMS


Atmospheric pressure drift tube ion mobility is a powerful addition to the Orbitrap mass spectrometer enabling direct separation of isomers by their size and shape. In this collaboration with CEA and Thermo Fisher Scientific, an Excellims MA3100 high resolution AP-DT-IMS was coupled to an Orbitrap to study the separation of metabolite and other biologically relevant isomers using drift gas mixtures for improved separation. Please follow the link below for the full free article:

To find out more about the MA3100, please click here for MA3100 product and applications information or contact our Sales & Applications team to schedule a demonstration:

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A new type of Cleaning Validation Analyzer

  • 30 seconds per sample, swab extract or rinse sample
  • Highly API specific and fully configurable
  • Small enough to be used at line
  • Easily operated in the field
  • Fully 21 CFR part 11 compliant

See the GA2200 cleaning validation analyzer in operation:


To find out more, please click here for GA2200 product and applications information or contact our Sales & Applications team to schedule a demonstration:

Contact Sales & Applications


Ion mobility offers high-speed, compound-specific detection with ppb-level sensitivity, making it very attractive for use in cleaning validation, where large numbers of samples need to be screened for API and detergents before production can continue.

Earlier implementations, however, required analytes to be volatile and thermally stable, gave only low analytical resolution or lacked 21 CFR part 11 compliant software, limiting the adoption of ion mobility for cleaning validation in pharmaceutical manufacturing.

The GA2200 overcomes all these limitations offering:

  • A versatile ESI source, able to ionize non-volatile and thermally sensitive compounds in a variety of different solvents (swab extract and rinse samples)

  • A high resolving atmospheric drift tube (R > 70), giving specific results for APIs and detergents in as little as 30 seconds per sample

  • All features required for full 21 CFR part 11 compliance including restricted individual user access, permissions management by user level, protected sample and method files, electronic signatures and a comprehensive electronic audit trail

The GA2200 thus enables a tenfold or higher increase in speed over HPLC-based methods while maintaining high sensitivity, good linearity and excellent repeatability:

The GA2200 is built for industrial environments with a compact, rugged design, easy- to-use touch screen and user levels from routine operator to full analytical method configuration mode.  

To find out more, please click here for GA2200 product and applications information. To schedule an instrument trial on your samples please contact our Sales and Applications team. We look forward to hearing from you!

Meet us in San Diego!

Interested in combining ion mobility with your Orbitrap or ion trap mass spectrometer? With the MA3100 you can add high resolution, plug & play IMS to your system in under 30 seconds! Applications include isomer separation (sugars, lipids, metabolites, vitamins), analysis of protein conformation states and peptide CCS, 2D characterization of complex mixtures, e.g. in petroleomics, and many more - please see below for a list of results presented at this AMS by our customers, collaboration partners and colleagues.

Intrigued? Please join us at booth 306 to take a look at the instrumentation and to discuss your application ideas or a potential instrument demonstration. We look forward to meeting with you!

Title Presenter Session
Deducing Association Energies from Shifts in Arrival Time Distributions: Impact of Selective Gas-Phase Ion-Vapor Clustering Pearl Kwantwi-Barima, Washington State University, Pullman, WA TOH pm 03:10
Separation of Saccharide Isomers using High Performance Ion Mobility Spectrometry on an Orbitrap Mass Spectrometer Aurelio La Rotta, Excellims Corporation, Acton MA TP 429
Separation of Noncovalently-Labeled Disaccharide Isobars by Traveling Wave and Frequency-Modulated Drift Tube Ion Mobility-Mass Spectrometry Kristin McKenna, Georgia Institute of Technology, Atlanta GA TP 410
Evaluating the influence of ion mobility techniques on conformer interconversion for routine quantitation of 25-hydroxyvitamin D3 Nicholas Oranzi, University of Florida, Gainesville FL TP 430
Separation of Isomeric Metabolites Using High Performance Ion Mobility in Various Drift Gases on an Orbitrap Mass Spectrometer Julia Kaszycki Excellims Corporation, Acton MA WP 432
Chirality Induced Gas-Phase Acidity and Conformational Changes in Cysteine-Polyalanine Peptides Yuntao Zhang, University of the Pacific, Stockton CA ThP 279
Investigation of the Gas Phase Structure of Polyproline Helix through Acidity Measurement Yadwinder Mann, University of the Pacific, Stockton CA ThP 280

How to add mobility to your MS in under 30 seconds

What if your mass spectrometer could also provide information on a molecule's 3D size and shape? Adding a mobility stage does just that, enabling you to:

  • Distinguish between isomers (e.g. oligosaccharides, lipids)
  • Analyze the conformation states of large molecules like proteins
  • Understand reaction pathways (charge location, molecular shape)
  • Increase confidence in identification through m/z plus CCS
  • ... and much more!

 If the above sounds interesting, you might want to consider adding a mobility stage to your MS - it is as simple as swapping out ion sources:


Easy to install and use, an add-on ion mobility stage is also inexpensive, as you already have the mass spectrometer in place. Interested? Please check out the MA3100 product pages or contact an application specialist using the link below!

Contact Sales & Applications


Excellims to exhibit and present at 65th ASMS Conference on Mass Spectrometry and Allied Topics, June 4 - 8, 2017 Indianapolis, Indiana. Visit us at booth #404 to meet our scientists and learn more about the high performance ion mobility spectrometry (HPIMS) products and the add-on HPIMS module for Thermo ScientificTM Orbitrap and other mass spectrometers.

Click here for the ASMS website and conference catalogue.

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