Separation of biologically relevant isomers on an Orbitrap mass spectrometer using high‐resolution drift tube ion mobility and varied drift gas mixtures
ion mobility, ion mobility spectrometry, high performance ion mobility spectrometry, biologically relevant isomers, Orbitrap, Thermo Fisher Scientific, API, Atmospheric pressure drift tube ion mobility
Atmospheric pressure drift tube ion mobility
Atmospheric pressure drift tube ion mobility is a powerful addition to the Orbitrap mass spectrometer, enabling direct separation of isomers. In addition to offering high resolving power in a compact design, it also facilitates optimization of the separation gas, as shown here for eight pairs of metabolites and gangliosides. About half of the separated compounds showed significantly better analytical separation when analyzed in a mixture of CO2 and air rather than air or CO2 alone.